Lipases and Phospholipases: Methods and Protocols

This third edition volume expands on the previous editions with an update on phospholipases, including structure-function relationships using the new tools of artificial intelligence, as well as current and new applications of both enzymes. The chapters in this book are organized into three parts: Part One provides readers with an overview on lipases and phospholipases; Part Two covers quantitative methods for assessing lipase activity and case studies on cloning and comparison of host for lipase expression; and Part Three presents relevant applications of lipases in new emerging fields such as nanobiocatalysis, polymers synthesis/degradation, and biosensors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Lipases and Phospholipases: Methods and Protocols, Third Edition is a practical and detailed reference for both graduate students and expert scientists. This book will be useful to the scientific community working with lipases, phospholipases, and related enzymes.