Apomixis: Methods and Protocols

This volume explores the latest research techniques (and reviews) used to study apomixis model species. The chapters in this book are organized into six parts: Part One introduces gametophytic apomixis, and Part Two focuses on the detection and characterization of apomixis. Part Three talks about transcriptomic profiling of apomictic and sexual species, and Part Four covers genetic analysis of apomixis. Part Five is dedicated to research on model apomictic systems such as dandelion (Taraxacum), Pilosella, Boechera, and Cenchrus (Pennisetum), and the final Part Six looks at protocols for engineering either individual components of apomixis, such as apomeiosis or a complete synthetic apomixis system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Apomixis: Methods and Protocols is a valuable reference for studying apomixis, and serves as an inspiration and foundation for designing new approaches and achieving significant breakthroughs in the field. Chapter 17 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.