CRISPR Delivery

This open access book explores the evolving landscape of genome editing delivery strategies, with a particular focus on CRISPR-based systems, offering a collection of current delivery approaches, ranging from viral vectors and lipid nanoparticles to physical methods such as electroporation, and presenting detailed protocols for the development and implementation of specific CRISPR/Cas delivery modalities. Beginning with the fundamental principles of CRISPR-based genome editing and the molecular and cellular machineries, the volume continues by covering the major delivery platforms, encompassing viral systems, such as lentiviral, adenoviral, and adeno-associated virus (AAV) vectors, as well as non-viral approaches, including lipid nanoparticles, electroporation-based delivery, extracellular vesicles, and other synthetic carriers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, CRISPR Delivery serves as a practical resource for researchers, clinicians, students, and industry professionals committed to improving CRISPR delivery and accelerating its translation into real-world applications. This publication is based upon work from COST Action Genome Editing for the Treatment of Human Disease Network (GenE-HumDi; COST Action CA21113), supported by COST (European Cooperation in Science and Technology). COST (European Cooperation in Science and Technology) is a funding agency for research and innovation networks. Our Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. www.cost.eu