Marburg Virus: Methods and Protocols

This volume explores the latest advancements in the study of the Marburg virus. The first few chapters in this book review the challenges associated with developing effective medical countermeasures against this virus, while the rest of the chapters provide protocols on ways to achieve these goals. Some of the topics covered in this book include virus isolation and propagation; single-cell transcriptional profiling; reverse genetics systems; proteomics analyses; next-generation sequencing techniques; protocols detailing the use of various animal models including bats and nonhuman primates; and methods for assessing virus stability or validating virus inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Marburg Virus: Methods and Protocols is a valuable resource for all researchers interested in advancing their understanding of this virus.