Advanced Mammalian Cell Culture Techniques: Principles and Practices

Provides step-wise protocols, flowcharts, key points to consider and troubleshooting tips Covers cellular staining using fluorescent dyes, genetic manipulation of cells via transfection, and an introduction to single-cell analyses Discusses basics in cell culture and downstream applications including gene and protein expression analysis Includes the principles underlying each of the techniques and provides a detailed methodology to practice Explores the whole range of techniques- from basic to downstream applications and advanced methods