Mammalian Oocyte Development: Methods and Protocols

This volume details various aspects of the very final stages of mouse oocyte development, and very early embryo development.  Chapters present methods ranging from in vitro growth of follicles, ewe oocytes, meiosis, identification of LADs (Lamin Associated Domains)/TADs (Topological Associated Domains), analysis of the oocyte, early embryo transcriptome, and mechanical characterization of these cells. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Mammalian Oocyte Development: Methods and Protocols, aims to be comprehensive guide for researchers in the field.